Assessment of Bioflocculant Production by Proteus vulgaris Isolated from Ikpoba River
Abstract
Bioflocculant production potential of freshwater bacteria isolated from
Ikpoba River where industrial effluents from brewery are been disposed
was investigated. The 16S rDNA sequence analysis revealed 86 %
similarity to Proteus vulgaris MF472597.1 and the nucleotide sequence
was deposited in GeneBank as Proteus vulgaris BKG3 with accession
number MK350331. Bioflocculant was optimally produced when
peptone (80.90 % flocculating activity) and glucose (81.00 %
flocculating activity) were used as nitrogen and carbon sources
respectively. The flocculating activity of the bioflocculant was
stimulated in the presence of Calcium Chloride (CaCl2). The highest
flocculating activities for kaolin clay suspension were achieved at pH
7.0 at a dosage of 0.16 mg/ mL. Chemical analyses of the purified
bioflocculant revealed that it was composed of 50 % carbohydrate and
4 % protein (wt/wt). The efficient flocculation capabilities of
bioflocculant produced by Proteus vulgaris MK350331suggest potential
use in wastewater treatment.