Quantitative Analysis of Phytochemical Constituents and Invitro Antioxidant Potentials of Poly-Herbal Formulation

Abstract

Plants of medicinal value have contributed to the well-being of many
people, individually and to the world at large. Vernonia amygdalina,
Greenwayodendron suaveolens, Euphorbia heterophylla and
Xylopia aethiopica are all known to have medicinal values varying
in their effect. The quantitative analysis of phytochemical
constituents and some invitro antioxidant properties of
Greenwayodendron suaveolens, Vernonia amygdalina, euphorbia
heterophylla and Xylopia aethiopica leaves were determined.
Phenols, flavonoids, tannins, saponins and tannins were the
phytochemicals detected from the plants. The determination of the
macro and micro elements such as iron, manganese, potassium,
calcium, magnesium, copper, lead, cadmium, chromium, and nickel
was made directly on each final solution using flame photometer and
a Bulk Scientific 210 VGP, atomic absorption spectroscopy (AAS).
The 1, 1-Diphenyl-2-picrylhydrazyl (DPPH), 2, 2-azino di-(3-
ethylbenzothiazoline- 6-sulfonic acid) (ABTS), hydroxyl free radical
(OH-) and ferric reducing antioxidant power (FRAP) were
investigated using one way analysis of variance (ANOVA), P<0.05
was obtained. In DPPH, ABTS, FRAP and OH- scavenging
activities, Vernonia amygdalina, Greenwayodendron suaveolens
and Euphorbia heterophylla was found to have scavenging activities
which increased as their concentrations increase. The medicinal
potential of these plants could be as a result of their phytochemicals,
micro and macro elements and their free radical scavenging
abilities. More investigation is necessary to be carried out to further
prove their anti-oxidative potentials