High-Performance Liquid Chromatography (HPLC) Profile and Anxiolytic Activity of Hexane Extract of Datura Metel Leaves Extract in Balb/c Mice

Abstract

Datura metel. family- Solanaceae, is a narcotic medicinal plant with various physiological effects. In this study, the anxiolytic activity in Balb/c mice and high performance liquid chromatography (HPLC) profile of the hexane extract of the leaves were investigated. The plant leaves were extracted in with hexane solvent in a soxhlet extractor apparatus for eight hourly period and concentrated using a rotary evaporator (Model RE, 200, USA). Phytochemical screening were done according to standard methods, flavonoid profile was assessed using HPLC analysis while anxiolytic activity conducted by the hole board method. Steroids, flavonoids, terpenoids were present in the extract except tannins. Scopolamine was detected as the highest flavonoid constituents in the extract. Group C which received the 200 mg/kg dose showed a positive anxiolytic activity (more head dippings) when compared with the standard control, diazepam. However, high anxiolytic activities were observed for groups B (100 mg/kg) and D (400 mg/kg) though not dose dependent while group A (negative control, distilled water) showed anxiogenic activity of increase in anxiety. The study revealed that D. Metel possess anxiolytic activity in Balb/c mice