Response Surface Optimization of Bioactive Compounds Extraction from Buchholzia coriacea Using D-Optimal Design

Abstract

This study investigates the use of D-optimal design and response
surface methodology for the optimization of bioactive compound
extraction from Buchholzia Coriacea. After thorough sample
preparation, the Soxhlet apparatus was employed to extract the
bioactive compound from the dried Buchholzia coriacea seed in a
batch-wise process. The D-optimal design was employed to generate
23 sets of experimental runs to determine the effect of temperature,
extraction time, and the weight of the dried sample on the extraction
yield. The temperature, extraction time, and the weight of the dried
sample were found to significantly influence the extraction yield
resulting in a maximum value of 41%. Optimum conditions of 80oC, 20
g, and 50 min representing temperature, the weight of the dried
sample, and extraction time were obtained using the desirability
analysis.