Bioactivity Assessment of Multi-doped Hydroxyapatite Nanoparticles Ca9.2Mg0.8Cu0.001(PO4)5.9(CO3)0.06(SiO4)0.04(OH)2.0 from Ion Release and Morphology Correlators

Abstract

This work focused on the bioactivity study of carbonated hydroxyapatite (Ca₁₀(PO₄)₆(CO₃)₆(OH)₂) and multi-doped (Mg²⁺, Si⁴⁺ and Cu²⁺) carbonated hydroxyapatite (Ca_10-x/2-a-bMg_aCu_b(PO₄)_6-x-y(CO₃)_x(SiO₄)_y(OH)_2-y).The nanopowders were cylindrically pressed into pellet and sintered at 800 °C and transferred into 30 mL vial Simulated Body Fluid (SBF) media for bioactivity analysis.  Materials characterization tools such as X-ray fluorescence (XRF), Field Emission Scanning Electron Microscope (FES-SEM) and Inductive Coupled Plasma Optical Emission Spectroscopy (ICP-OES) were used for elemental composition, ion release and morphology studies respectively. The obtained result indicate that the multi-doped sample tagged MSC-CHAp shows a superior bioactivity with visible apatite layer compared to the control sample (CHAp). This improved bone-like apatite layer was due to lower ion release in the solution and higher participatory activity of Ca²⁺ and PO₃^4- on the surface of the pellets.